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. 2017 Feb 24;13(2):e1006642. doi: 10.1371/journal.pgen.1006642

Fig 4. Expression patterns of SID1 and its transcriptional activation.

Fig 4

(A) Transcript levels of SID1 in the indicated organs at seedling stage. The data are means ± SEM of three independent experiments. (B–H) GUS staining (blue) of distinct organs in pSID1::GUS transgenic plants: (B) root; (C) expanding leaf; (D) young leaf; (E) sheath; (F) longitudinal section of the shoot apical meristem; (G) stem; (H) floret. Scale bar, 2 mm. (I) Subcellular localization of SID1. Full-length SID1 fused with green fluorescent protein (GFP); the nuclear protein Ghd7 fused with cyan fluorescent protein (CFP) served as a nuclear marker. The two proteins were co-expressed in rice protoplasts. A bright-field image and the merged image are shown on the right. Scale bar, 10 μm. (J) Relative luciferase activities of rice protoplasts co-transfected with reporter and distinct effectors. Schemes of deletion mutants of SID1 are shown at left. Gray bars in the N-terminal region of SID1 indicate four zinc finger motifs. All luciferase activities are expressed relative to the value of GAL4 BD alone. Values represent means of three independent experiments.