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. 2017 Mar 10;12(3):e0173716. doi: 10.1371/journal.pone.0173716

Fig 7. Photoreceptor degeneration in aged Cxcr5-/- mice.

Fig 7

17-month-old C57BL/6 WT mice and Cxcr5 KO mice were used. (A and B) Representative toludin blue-stained images of the WT mice (A) and the KO mice (B). (C) Quantification of ONL thickness. The superior retinal areas that were near optical nerve head approximately 100 micron were used for ONL thickness measurement. The values were the mean (± SD; n = 10). (D and E) Representative TUNEL staining images of the WT mice (C) and the KO mice (D). Arrows indicated the TUNEL (+) cells in ONL layer. (F) Quantification of TUNEL (+) cells (mean ± SD; n = 6). (G) Representative active Caspase-3 staining images of the KO mice. The merged picture with DAPI staining showed the localization of the active caspase-3 in photoreceptor inner segments. Arrows indicated active Caspase-3 staining signals. (H and I) A-wave (H) and b-wave (I) amplitudes of scotopic ERG. The values were the mean (± SD; n = 10). * denoted p <0.05. GCL: ganglion cell layer; IPL: Inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; PIS: photoreceptor inner segment; POS: photoreceptor outer segment; RPE: retinal pigment epithelium.