(A) Double bond index/saturated FA ratios (DBI/sat) for PC, PE and PI. (B) Lipid species compositional alterations for major membrane lipids PC, PE, and PI in the WT strain. (C) Changes in PC/PE ratios. (D) Changes in the contribution of medium chain FA-containing species to membrane lipid composition. (E) Alterations in lyso-lipid species. Cells were untreated (30°C) or stressed at 40°C for 1 h. Values are expressed as mol% of MLs (mean ± SD), n = 3 for plh1Δ and dga1Δ, n = 4 for DKO, and n = 7 for WT; * p<0.05 (30°C vs 40°C), # p<0.05 (WT vs mutants at 30°C), $ p<0.05 (WT vs mutants at 40°C). DBI/sat, double bond index/saturated FA ratio; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol.