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. 2017 Mar 10;12(3):e0173866. doi: 10.1371/journal.pone.0173866

Fig 1. Dectin-1 alone is not sufficient to bind C. neoformans spores.

Fig 1

CHO-K1 cells were engineered to express murine Dectin-1 (Clec-7a) with a C-terminal HA tag. Dectin-1-HA protein expression and localization were verified with an antibody directed against HA and conjugated to Cy3 (red). Visual assays were used to assess binding of heat-killed C. albicans yeast (A) and heat-killed C. neoformans spores (B). Fungal cells were stained with calcofluor white (blue). Cells were evaluated using both light and fluorescence microscopy at 1000X magnification. White bars represent 10 μm. (C) Interactions between CHO cells and live yeast or live spores were quantified in a representative biological replicate (1 of 3) by assessing a minimum of 300 CHO cells (7–10 frames) per condition, and the change in association was compared in the presence or absence of Dectin-1-HA for each fungal cell type using a Fisher’s Exact Test for statistical analysis (bracket values). X-axis shows fungal cell types. Y-axis represents % CHO cells associated with fungi. Black bars represent Dectin-1-HA expressing cells. Gray bars represent cells harboring an empty expression vector (EV).