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. 2017 Feb 24;13(2):e1006638. doi: 10.1371/journal.pgen.1006638

Fig 3. Chromosomal movement reported by DAPI and HupA-mCherry labels.

Fig 3

A: Time-lapse images showing DAPI (top row) and HupA-mCherry labelled chromosomes (middle row). Arrows point to location of cell division plane. Phase images for the same cell are shown in the bottom row. Scale bar is 2 μm. B: Normalized intensity from DAPI and HupA-mCherry labels during cell division for the cell shown in panel A. Dashed horizontal line corresponds to the expected value for both intensities at the end of the division. C: Initial normalized intensity vs final normalized intensity from DAPI label (N = 46). Dashed lines correspond to the expected values for the final normalized intensity. In this plot data from cells without Z-ring marker (strain JM30) and with induced ZipA-GFP Z-ring marker (strain MB16) have been combined. The data from two strains are plotted separately in S3 Fig in Supplemental Materials. These data show no significant differences between the two strains.