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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: J Am Chem Soc. 2017 Feb 13;139(7):2541–2544. doi: 10.1021/jacs.6b10792

Figure 1.

Figure 1

A) Thiocillin gene cluster with genomic deletion of endogenous prepeptide genes tclE-H, B. cereus ATCC 14579 ΔtclE-H. B) A plasmid complementation method to generate site-saturation mutagenesis libraries at residues 29 of the macrocycle. LP = leader peptide.