Table 2.

Cells detached into supernatant after controlled trypsinization.

T, min	10	30	60
NCI-H226 Cells
Control (number of cells in supernatant)	116 950	195 616	245 266
Treatment (number of cells in supernatant)	Not detected	Not detected	Not detected
B16-F10 cells
Control (number of cells in supernatant)	479 400	537 050
Treatment (number of cells in supernatant)	Not detected	Not detected

Abbreviation: RPMI, Roswell Park Memorial Institute.

Cells were treated as described in Figure 1A. At each time point, the supernatant in the well was collected into 0.5 mL of RPMI 1640 medium, centrifuged, resuspended in 0.1 mL of RPMI medium, and counted by a Cellometer Auto T4 Plus Cell Counter. Data presented were from 1 of the 3 representative experiments.