Table 3.

Occurrence of spirorchiids in each organ, by genera and by species.

	Aorta	Bladder	Brain	FP	GB	GIT	Gonads	Heart	Kidney	Liver	Lung	Pancreas	Spleen	Thyroid
Total samples	2	2	43	2	2	16	3	12	14	11	14	8	12	7
PCR positive (%)
Hapalotrema	100	0	37	100	100	56	0	50	29	55	57	75	75	71
Carettacola	0	0	5	0	0	13	0	0	0	18	0	0	17	0
Neospirorchis	100	100	93	100	50	88	100	100	100	82	93	100	100	100
Total T-RFLP tested - Hapalotrema	2	2	39	2	2	13	3	12	14	11	13	8	12	6
T-RFLP Positive (%)
Hapalotrema postorchis	50	0	13	0	50	46	0	25	21	36	15	50	50	33
Hapalotrema pambanensis	50	0	21	50	50	31	0	33	14	27	31	50	58	33
Hapalotrema synorchis	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Learedius learedi	0	0	15	50	50	8	0	17	7	9	23	25	17	0
Hapalotrema mistroides	0	0	0	0	50	0	0	8	0	9	15	0	25	33
Amphiorchis sp.	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Total T-RFLP tested - Carettacola	2	2	41	2	2	16	3	12	14	11	14	8	11	7
T-RFLP Positive (%)
Carettacola hawaiiensis	0	0	0	0	0	13	0	0	0	18	0	0	9	0
Carettacola Gen. 2	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Total T-RFLP tested - Neospirorchis	2	2	43	2	2	16	3	10	14	10	14	8	11	7
T-RFLP Positive (%)
Neospirorchis Gen. 1	50	0	30	50	0	19	33	50	43	20	21	50	27	43
Neospirorchis Gen. 2	100	100	95	100	50	88	100	100	100	80	93	100	100	100
Neospirorchis Gen. 3	0	0	0	0	0	6	0	0	21	0	50	0	9	0
Average no. Hapalotrema spp. present	1	0	0	1	2	1	0	1	0	1	1	1	2	1
Average no. Carettacola spp. present	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Average no. Neospirorchis spp. present	2	1	1	2	1	1	1	2	2	1	2	2	1	1
Average total species present	2.5	1	1.8	2.5	2.5	2.2	1.3	2.1	2.1	2	2.5	2.8	3.1	2.5

Total samples refers to total samples available for molecular testing. Total PCR positive refers to number of samples that were positive for each genus/group of genera based on first round multiplex PCR results. 'Total T-RFLP results' columns refer to total number of samples that were successfully characterised by T-RFLP, or had a validated negative result on multiplex PCR. Abbreviations: GIT - Gastrointestinal tract, GB = Gall bladder, FP = Fibropapilloma.