Fig. 4.

Effects of Xn treatment on LPS-activated Txinp-NLRP3 inflammasome and NF-κB signal pathways in ALI mice. Mice were given different concentrations of Xn (10 or 50 mg/kg) with an intraperitoneal injection 1 h prior to LPS (0.5 mg/kg) pretreatment and lungs of mice were collected. Protein samples were extracted from the lungs and analyzed by Western blot with specific antibodies. (A and D) Protein expressions of Txnip, Trx-1, NLRP3, ASC, caspase-1 and IL-1β were measured by Western blot analysis. (G) Protein expressions of P-p65, p65, P-IκBα, and IκBα were measured by Western blot analysis. (B, C, E, F, H and I) Quantification of relative protein expression was performed by densitometric analysis and β-actin was acted as an internal control. Similar results were obtained from three independent experiments. All data are presented as means±SEM (n=5 in each group). ^##p<0.01 vs Control group; ^*p<0.05 and ^**p<0.01 vs LPS group.