Fig. 2.

Effect of PCB-153 on lipid accumulation in vivo and in vitro. Mice were fed high fat diet (HFD) and drank water containing PCB-153. At the end of the experiment, serum (A) and liver (B) triglyceride (TG) content was determined to assess lipid accumulation in circulation and livers. Epididymal fat weight was weighed (C) and HE staining of adipose was conducted (D) to evaluate lipid accumulation in adipose tissue. *p<0.05, significant differences between the two groups. AML-12 hepatocytes were treated by 0.5 and 1 μM PCB-153 for 24 h and cells were stained with BODIPY to observe lipid droplets (E). TG content was also determined in hepatocytes (F). Differentiated 3T3-L1 adipocytes were treated by 0.5 and 1 μM PCB-153 for 24 h and cells were stained with BODIPY to observe lipid droplets (G). TG content was also determined in hepatocytes (H). *p<0.05, significant differences compared with control.