Fig. 5.

Effect of PCB-153 on oxidative stress in vivo and in vitro. Mice were fed high fat diet (HFD) and drank water containing PCB-153. At the end of the experiment, frozen sections of livers (A) and adipose tissues (B) were cut and stained with 10 μM dihydroethidium (DHE) to evaluate ROS level. Representative images were captured. AML-12 hepatocytes and differentiated 3T3-L1 adipocytes were treated by 0.5 and 1 μM PCB-153 for 24 h. After that, cells were incubated with 10 μM DHE and analyzed using flow cytometry. ROS level was expressed as folds of control (C and D). 0, 3, 6, 12, and 24 h after the treatment of PCB-153 in hepatocytes, ROS level was determined by DHE staining (E). 24 h after the treatment of PCB-153 in hepatocytes, GSH, GSSG, NADP+ and NADPH content were determined and GSH/GSSG ratio and NADP+/NADPH ratio were shown (F and G). *p<0.05, significant differences compared with control.