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Fluorescence labeling of FAK in human skin fibroblasts exposed for 24 hours to 25 mM, 50 mM, or 100 mM ethanol.
Notes: Pictures of fluorescing cells from microscope Nicon Eclipse Ti/C Plus. (A) Cellular nuclei stained with Hoechst dye (green). (B) Immunodetection of FAK using antibodies to FAK labeled with FITC (red). (C) Overlapped pictures with labeled cellular nuclei and with labeled FAK. Scale bar =20 µm.
Abbreviations: FAK, focal adhesion kinase; FITC, fluorescein isocyanate.
