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. 2017 Mar 6;11:669–676. doi: 10.2147/DDDT.S125843

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© 2017 Donejko et al. This work is published and licensed by Dove Medical Press Limited

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Fluorescence labeling of FAK in human skin fibroblasts exposed to 25 mM, 50 mM, or 100 mM ethanol +500 µg/mL HA.

Notes: Pictures of fluorescing cells from microscope Nicon Eclipse Ti/C Plus. (A) Cellular nuclei stained with Hoechst dye (green). (B) Immunodetection of FAK using antibodies to FAK labeled with FITC (red). (C) Overlapped pictures with labeled cellular nuclei and with labeled FAK. Scale bar =20 µm.

Abbreviations: FAK, focal adhesion kinase; FITC, fluorescein isocyanate; HA, hyaluronic acid.