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. 2017 Mar 3;6:21–30. doi: 10.2147/OV.S123292

Table 1.

Conditions of normal and tumor cells

Cell name Code Tissue Species Growth medium (+10% FBS) Culture condition
Tumor
A549 RCB0098 Non-small cell lung carcinoma Human F12-K Adherent, 37°C, 5% CO2
U87MG HTB14 Glioblastoma multiforme Human High glucose DMEM Adherent, 37°C, 5% CO2
T98G RCB1954 Glioblastoma multiforme Human RPMI-1640 Adherent, 37°C, 5% CO2
Huh7 RCB1942 Hepatocellular carcinoma Human High glucose DMEM Adherent, 37°C, 5% CO2
MDA-MB-453 RCB1192 Mammary gland adenocarcinoma Human Leibovitz-15 Adherent, 37°C, no CO2
MCF7 RCB1904 Mammary gland adenocarcinoma Human MEM +0.1 mM NEAA +1 mM sodium pyruvate Adherent, 37°C, 5% CO2
Normal (nonmalignant)
HEK293 RCB1637 Human embryonic kidney Human High glucose DMEM Semi-adherent, 37°C, 5% CO2
Monocytes Adherent mononuclear cells (primary) Human RPMI-1640 Adherent, 37°C, 5% CO2
NB1RGB RCB0222 Normal skin fibroblast Human MEMα Adherent, 37°C, 5% CO2
HF19 RCB0210 Normal lung fibroblast Human MEMα Adherent, 37°C, 5% CO2
RCR-1 RCB0075 Astroglia Rat DMEM Semi-adherent, 37°C, 5% CO2

Abbreviations: DMEM, Dulbecco’s Minimal Essential Medium; FBS, fetal bovine serum; F12-K, Kaighn’s modification F12 nutrient medium; MEM, minimal essential medium; NEAA, nonessential amino acids; RPMI-1640, Roswell Park Memorial Institute-1640.