Effects of MA (10 mg/kg i.p. every 2 hrs ×4), EtOH (6 g/kg), and CEF (200 mg/kg) on GLT-1, xCT and GLAST in EtOH groups in both striatum and hippocampus. (A, C, and E) Immunoblots for GLT-1, xCT and GLAST, respectively. (B) Quantitative analysis showed a significant increase in the ratio of GLT-1/β-tubulin in EtOH-MA-CEF groups compared to EtOH-MA-SA group in striatum and hippocampus. A significant downregulation of GLT-1 expression was found in EtOH-MA-SA groups compared to control EtOH-SA-SA groups in both brain regions. No significant difference in GLT-1 expression was found in EtOH-MA-CEF group compared to EtOH-SA control group. (D) Quantitative analysis did not reveal any significant difference in the ratio of xCT/β-tubulin between all tested groups in both brain regions. (F) Quantitative analysis did not reveal any significant difference in the ratio of GLAST/β-tubulin between all tested groups in both brain regions. Values shown as means ± S.E.M (*p < 0.05) (n=6–7 for each group).