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. Author manuscript; available in PMC: 2018 May 5.
Published in final edited form as: Spectrochim Acta A Mol Biomol Spectrosc. 2017 Feb 2;178:185–191. doi: 10.1016/j.saa.2017.01.069

Figure 2.

Figure 2

Difference between two signal averaged D2O reference transients. (A) Difference IR absorbance transient of 20 mM potassium phosphate buffer (pD 7) detected at 1619 cm−1 with a 1000 shot average. (B) Difference fluorescence transient of 400 μM Trp in 20 mM potassium phosphate buffer (pD 7) excited at 285 nm and monitored at 350 nm using a 200 Ω resistor and a 5000 shot average.