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. 2004 Nov 29;101(49):17126–17131. doi: 10.1073/pnas.0407492101

Fig. 2.

Fig. 2.

Striatin targets ERα to the plasma membrane. (A–C) EAhy926 cells without overexpression of striatin were immunostained for ERα (A) or striatin (B), and the images were merged (C). Under these conditions only minimal amounts of membrane-associated ERα is detected. (D–I) EAhy926 cells transfected with a striatin expression plasmid were immunostained for ERα (A and D), striatin (B, E, and H), or eNOS (G), and the images were merged (C, F, and I). Under these conditions substantial membrane-associated ERα is detected, which colocalizes with striatin, as does eNOS. (J) Overexpression of striatin increases the proportion of ERα detectable in membrane fractions. Lysates of EAhy926 cells transfected with a control vector (–) or a striatin expression plasmid (+) were fractionated by differential centrifugation. The purity of plasma membrane and nuclear fractions was identified by immunoblotting for predominantly membrane proteins [EGFR and IGFR (data not shown)] or the nuclear protein histone.