Fig. 1.

Alignment of DP71L with domains from GADD34 and ICP34.5 and mutants of DP71L Panel A) Shows an alignment of the long and short forms of DP71L with the C terminal domain of ICP34.5 of HSV-1 and GADD34. Within the C terminal region of ICP34.5 residues 233–248 (green) have been identified as the eIF2α binding domain (Li et al., 2011), whilst the eIF2α binding motif described by Rojas et al. (2015) in GADD34 is shown in blue. The predicted PP1 binding motif is highlighted in red. Panel B) shows the sequences of mutants of DP71L generated in this work. Numbers denote positions within wild type short DP71L sequences that correspond to mutations made. Dashed lines indicate the sequence is not altered from the wild type sequence and gaps show sequences deleted.