Fig. 4.

DP71L mutant V16E, F18L does not co-precipitate with PP1 Vero cells were transfected with plasmids expressing wild type or mutant DP71L, 24 h post-transfection lysates were harvested and incubated overnight with the HA affinity matrix at 4 °C with rotation. Lysates were pelleted and washed three times prior to re-suspension in SDS-PAGE loading buffer. Samples were resolved by SDS-PAGE, transferred to membranes by Western blot and probed against the HA epitope tag and PP1.