Fig. 5.

The V16E; F18L form of DP71L is unable to interact with protein phosphatase isoforms Panel B. Yeast strain PJ69-4α was transformed with pairs of plasmids expressing the indicated DNA binding hybrid and the phosphatase (PPC1) isoform fused to the yeast GAL4 activation domain. Yeast containing both plasmids were selected on synthetic drop-out medium lacking leucine and tryptophan (“-LW”) and then streaked onto synthetic drop-out medium lacking leucine, tryptophan and histidine and containing 5mJM or 10 mM 3-aminotriazole (“-LWH +3AT”). Growth on the latter is indicative of protein-protein interaction.