Fig. 6.

Wild type, but not mutant, DP71L acts as a translation enhancer Vero cells were co-transfected with equal amounts of the bi-cistronic reporter plasmid pIRES FF luc/Ren luc and pcDNA3, wild type or mutant DP71L as indicated. 24 h post-transfection cells were lysed and reporter activity assessed using the Dual-Luciferase Reporter Assay kit (Promega). The firefly (A) or renilla (B) reporter activity of control cells transfected with pcDNA3 was set at 100% and wild type or mutant activity expressed as a percentage relative to pcDNA3. Experiments were performed in triplicate three times. Error bars represent the standard deviation. Statistical analysis was carried out in GraphPad Prism using a one way ANOVA with multiple comparisons test. Asterisks represent a significant difference in value between WT DP71L and the mutants tested (*= P value of <0.5, ****= P value of <0.0001).