Table 1.
Effect of wild type and mutant forms of DP71L on CHOP induction.
Plasmids expressing wild type or mutant forms of DP71L were transfected into Vero cells. At 24 h post-transfection cells were stimulated with 20 µg/ml tunicamycin for 8 h to induce expression of CHOP. Cells were then fixed in 4% PFA, permeabilised and labelled with DAPI, anti-HA and anti-CHOP antibodies. Confocal microscopy was used to visualise 150–200 cells expressing the DP71L proteins and determine the percentage of those in which nuclear localisation of CHOP was induced. The first column shows the DP71L wild type or mutant proteins tested. The second column indicates the numbers of transfected cells in which CHOP nuclear localisation was detected. The – symbol indicates none and + or ++ increasing numbers of cells with CHOP detected in the nucleus. The third column expresses the percentages of transfected cells in which CHOP was detected in the nucleus.
| CHOP Induction | % CHOP inhibition | |
|---|---|---|
| DP71Ls | – | 98 |
| V16E, F18L | ++ | 17 |
| Δ52–66 | ++ | 13 |
| Δ52–61 | ++ | 23 |
| Δ57–66 | ++ | 18 |
| ΔLSAVL | + | 39 |
| L57A | + | 31 |
| S58A | – | 98 |
| V60A | – | 98 |
| L61A | +/- | 57 |
| L57,61A | + | 25 |
| V16E, F18L, L57,61A | ++ | 20 |
| V16E, F18L, ΔLSAVL | + | 31 |