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The NGS library preparation procedure. Genomic DNA is fragmented to 100–500 bp by sonication or enzyme digestion. The fragmented DNA is end-repaired to generate a blunt end. An additional dAMP is incorporated into the 3′ end of a blunt DNA fragment. The Y-shaped adapter is ligated to both ends of DNA. The ligated DNA is amplified by PCR to generate enough materials for sequencing.
