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Five kinds of barcoded beads for RNA sequencing. mRNA is captured by ploy-T tail and reverse transcribed. (a) The bead structure was adapted from CEL-seq.95 Each cell was barcoded before linear amplification. (b) UMI was incorporated to reduce amplification artifacts. (c) The beads used in the Drop-seq protocol which took advantage of template switching.103 (d) The beads used by Fan et al. to generate sequencing-ready libraries. (e) The beads used in the Hi-SCL protocol.101
