TABLE I.
Currently available microfluidic approach for genome wide analysis.
| Field | Approach | Reference | Format | Comment |
|---|---|---|---|---|
| Genomics | Digital PCR | 56 | Micro-chamber | 1176 × 12 parallel reactions |
| MDA | 48, 49 | Micro-chamber | Single cell, 9 parallel reactions | |
| 50, 51 | C1 | Single cell, 96 parallel reactions | ||
| 46, 66 | Droplet | Single cell | ||
| 52 | Micro-well | Single cell, ∼400 cells per run | ||
| 53 | Gel | Single cell | ||
| MALBAC | 55 | Micro-chamber | Single cell, 8 parallel reactions | |
| PicoPLEX | 54 | Droplet | Single cell, 95 parallel reactions | |
| Transcriptomics | T7 linear amplification | 91 | Micro-chamber | 20 pg to 10 ng purified RNA |
| SMART-seq | 112–114 | C1 | Single cell, 96 parallel reactions | |
| Drop-seq | 103 | Droplet | Single cell, ≥1000 cells each run | |
| Hi-SCL | 101 | Droplet | Single cell, 10 000 cells in 4.3 h | |
| inDrop | 102 | Droplet | Single cell, ≥ 3000 cells | |
| Single-cell RNA printing and sequencing | 100 | Micro-well | Single cell, 600 cells | |
| CytoSeq | 99 | Micro-well | Single cell, up to 10 000 cells each run | |
| Tang's protocol94 | 107 | Micro-chamber | Single cell, 8 parallel reactions | |
| Epigenomics | ChIP-seq | 128 | Micro-chamber | 1000 cells |
| MOWChIP-seq | 129 | Micro-chamber | 100 cells | |
| Drop-ChIP | 131 | Droplet | Single cell, 100 cells each run | |
| Transcription factors Binding affinity | 156–161 | Micro-chamber | 2400 parallel reactions | |
| ATAC-seq | 130 | C1 | Single cell, 96 parallel reactions |