Table 1.
NOS2 overexpression and shRNA-knockdown viral vectors
| Human NOS2 overexpression | Vector construct | Company |
|---|---|---|
| Control vector (empty vector) | pLenti6-MCS-GFP-2 A-Puro | N/A |
| Human NOS2 vector (Accession BC130283) | pLenti-GIII-CMV-Human NOS2-GFP-2 A-Puro | abm® inc. |
| Human NOS2 shRNA knockdown | Vector construct | Company |
| Control shRNA (non-target control shRNA) | pLenti6-U6-NT shRNA-GFP-2 A-Puro2 | N/A |
| NOS2 Mission® TRC shRNA (shRNA 1) | Mission shNOS2 (TRCN0000231570) | Sigma-Aldrich® |
| NOS2 Mission® TRC shRNA (shRNA 2) | Mission shNOS2 (TRCN0000231573) | Sigma-Aldrich® |