Fig. 2.

Assessment of extraction methods for total protein analysis. Different methods for extracting total protein for subsequent Lowry analysis were compared. Freeze-dried and finely ground algal biomass was subjected to extraction by (1) water, room temperature, 1 h; (2) water, pH 11, room temperature, 1 h; (3) water, 80 °C, 1 h; (4) 2 % SDS + 1 mM dithiothreitol (DTT), 100 °C, 3 × 5 min; (5) 2 % SDS + 1 mM DTT, pH 11, 100 °C, 3 × 5 min; and (6) 2 % SDS + 1 mM DTT, pH 11, 100 °C, 3 × 5 min in two steps. The same biomass was analyzed with (7) Kjeldahl method, for comparison