Table 1. Levels of mitochondrial transcripts 5 h after transient transfection of HEK cells treated for 4 days with EBr or untreated control cells.
| n | RNA19/18S rRNA | ND1 mRNA/18S rRNA | |
|---|---|---|---|
| Untreated control | 5 | 0.73 ± 0.04 | 1.09 ± 0.25 |
| 4 d EBr | 5 | n.d. | n.d. |
| Untransfected | 5 | 0.15 ± 0.05 | 0.30 ± 0.01 |
| pKEX | 10 | 0.16 ± 0.02 | 0.38 ± 0.01 |
| pKEX-TFAM | 10 | 0.22 ± 0.04 | 0.50 ± 0.14 |
| pKEX-ΔC-15-TFAM | 10 | 0.18 ± 0.06 | 0.49 ± 0.18 |
n, number of individual plates; n.d., not detectable; values are mean ± SD.
Cells remained untransfected or were transfected with the indicated plasmids.