Table 1. Results of sequence analysis of the 3′ ends of selected mitochondrial RNAs.
| Mitochondrial RNA | Number of clones analyzed | Analysis of the poly(A) tails | ||
|---|---|---|---|---|
| The sequence of the 3′ end | Average length | Ratio A:G/C | ||
| ND3 | 6 | (A)18; (A)33; (A)45; (A)47; (A)58 | 40 ± 14 | 238:1 |
| (A)14 G(A)23 | ||||
| ATP6/8 | 17 | (A)13; (A)16; (A)29; (A)34; (A)42; (A)44; (A)45; (A)46; (A)48; (A)50; (A)51; (A)62; | 42 ± 13 | 709:10 |
| (A)8 C(A)34 | ||||
| (A)9 C(A)38 | ||||
| (A)13 CC(A)38 | ||||
| (A)39 CC(A)5 | ||||
| (A)25 CCAGG(A)19 | ||||
| COXIII | 17 | (A)10; (A)14; (A)17; (A)49; (A)49; (A)50; (A)51; (A)51; (A)52; (A)53; (A)56; | 47 ± 16 | 801:0 |
| (A)56; (A)58; (A)58; (A)58; (A)58; (A)61 | ||||
| CYTB | 6 | (A)0; (A)0; (A)0; | a | |
| (A)29; (A)36; (A)24 C(A)19 | 36 ± 8 | 108:1 | ||
| 12S rRNA | 5 | (A)1; (A)1; (A)1; (A)2; (A)2 | a | 7:0 |
| Total | 51 | 43 ± 14 | 1863:12 |
(A)n indicates the stretch of n adenines.
(A)0 means no poly(A) tail; the clones probably represent degradation intermediates as they lack mtDNA-encoded nucleotides at their 3′ termini.
aNot taken into account during calculations.