REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Endura competent E. Coli cells	Lucigen	60242-0
Feeding bacteria HT115 E. Coli cells	Gift from Eric Meyer (ENS, Paris)	HT115
Klebsiella pneumoniae non-virulent strain, food source for Paramecium	Gift from Eric Meyer (ENS, Paris)	N/A
Chemicals, Peptides, and Recombinant Proteins
Wheat Grass Powder	Pines International, Lawrence, KS	N/A
β-sitosterol	Calbiochem, Millipore	CAS 83-46-5
		567152
T4 DNA Ligase	New England Biolabs	M0202
Critical Commercial Assays
TRI Reagent	Sigma-Aldrich	T3809
GoScript Reverse Transcription kit	Promega	A5000
GoTaq G2 DNA polymerase	Promega	M8296
Wizard® SV Gel and PCR Clean-Up System	Promega	A9281
pGEM®-T Easy Vector System	Promega	A3600
Illumina TruSeq small RNA kit	Illumina	RS-200-0012
Deposited Data
Ligase IV silencing and empty vector control silencing small RNA data	This work	GenBank, accession number ENA: SUB2107094
Experimental Models: Organisms/Strains
Paramecium tetraurelia strain 51	Gift from Eric Meyer (ENS, Paris)	N/A
Oligonucleotides
See Table S1 for complete list of oligonucleotides	This paper	N/A
Recombinant DNA
Plasmid L440 for feeding bacteria	Gift from Eric Meyer (ENS, Paris)	N/A
Dcl5 silencing construct	Sandoval et al., 2014	N/A
Ligase IV silencing constructs	This work, see Table S1	N/A
Software and Algorithms
Geneious 8.1.8	http://www.geneious.com/	N/A