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. 2016 Dec 29;101(4):1037–1044. doi: 10.1189/jlb.3A0916-221RR

Figure 3. IL-10+ hepatic CD8+ T cells have an activated phenotype consistent with terminally differentiated effectors.

Figure 3.

Phenotype of hepatic CD8+ T cells from TLR9–MAS IL-10 reporter mice. (A) Representative histograms showing expression of surface markers differentiating naïve, effector, and memory populations. Plots are gated on CD8+ T cells, either in total (for PBS conditions) or IL-10/GFP− and IL-10/GFP+ subsets (for CpG conditions). (B) Summary of data as shown in (A). n = 4 mice/group, either pooled from 2 independent experiments (CD44, CD62L) or representative of 2 independent experiments (CD69, KLRG1, CD127). (C) Representative histograms and summary data for T bet and Eomesodermin expression. Plots are gated on CD44+CD8+ T cells. Naïve and effector splenic CD8+ T cells are included for comparison. N = 4 paired samples per group. Data are representative of 2 independent experiments. Analyzed by paired Student’s t test. MFI, mean fluorescent intensity. *P < 0.05, *** P < 0.001.