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. 2017 Mar 11;17:59. doi: 10.1186/s12866-017-0965-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Transcriptional analyses of putative A. baumannii Zn and Cu efflux systems. The mRNA transcription levels of the putative (a) Cation Diffusion Facilitator (CDF), (b) Heavy Metal Efflux (HME), (c) P-type ATPase, (d) CorA-type and (e) CopB-like copper resistance genes of A. baumannii strain ATCC 17978 were determined by qRT-PCR. Transcription levels were examined in the presence of 400 μM Zn, 400 μM Cu or 400 μM Zn + 400 μM Cu and corrected to untreated cells following internal normalisation to GAPDH. The data are the mean of at least biological triplicates (± SEM). Statistical analyses were performed by one-way ANOVA using Dunnett’s posttest; ns = not significant, * = p < 0.05, ** = p < 0.01, *** = p < 0.001 and **** = p < 0.0001