Table 2.

Characterization of the physico-chemical properties of polymer brushes on scaffold focusing on factors that can affect cell behaviors

	Wettability	Roughness	Rigidity/elasticity	Chemical composition	Height
Tools for analysis	Contact angle measurement	AFM, SEM	AFM (force mode)	IR & Raman spectrometery, XPS, TGA	Ellipsometry, AFM
Parameters	Angle between surface of a liquid drop and substrate	Root mean square of the height of surface contour	The degree of deflection of AFM cantilever at contact point	Chemical functional group, element composition, thermal decomposition	Height from the surface of scaffold
Properties	Hydrophobicity & hydrophilicity	Topology & geometry	Mechanical strength	Presence of target polymers on the surface	Thickness of polymer brush