Fig. 2.

Effects of transduced Tat-NQO1 protein against H₂O₂-induced cellular toxicity. Cellular distribution of transduced Tat-NQO1 protein in HT-22 cells. Cells were treated with Tat-NQO1 (3 μM) protein for 2 h, and the cellular distribution of Tat-NQO1 proteins was observed by confocal microscopy (A). Scale bar = 20 μm. Effect of Tat-NQO1 protein against H₂O₂-induced cellular toxicities. Tat-NQO1 proteins were added to the culture medium, after which cells were exposed to H₂O₂, as described in the “Materials and methods” section. Cell viability was assessed by MTT assay (B), ROS levels were measured using DCF-DA staining (C), and DNA fragmentation was detected by TUNEL staining (D). Scale bar = 50 μm. **P < 0.01, compared with H₂O₂-treated cells.