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. 2017 Feb 26;2017:5858315. doi: 10.1155/2017/5858315

Figure 2.

Figure 2

BNP activates NPR-1 in THP-1 cells. (a) THP-1 cells were treated with 10−8 M BNP in absence or presence of 10 μg/mL LPS/5 mM ATP and cGMP intracellular levels were evaluated after 5 and 30 min treatment. (b) THP-1 cells were treated with 10−8 M BNP in absence or presence of 10 μg/mL LPS/5 mM ATP for 30 min. Cell lysates were immunoblotted for NPR-1. The blots were stripped of the bound Ab and reprobed with mouse anti-β-actin, to confirm equal loading. Western blots are representative of three separate experiments. Histograms indicate means ± SD of three separate experiments each one tested in triplicate. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus untreated cells.