Figure 5.

NF-kB and ERK 1/2 are involved in NALP3/ASC/Caspase-1 cascade activation in THP-1 cells. THP-1 cells were incubated with 10 μM BAY 11-7082 (a, b, c) or U0-126 (d, e, f) for 1 h and then treated with 10 μg/mL LPS/5 mM ATP for 30 min. Cell lysates were immunoblotted for NALP3 (a, d), ASC (b, e), or Caspase-1 (c, f). The blots were stripped of the bound Ab and reprobed with mouse anti-β-actin, to confirm equal loading. Western blots are representative of three separate experiments. Histograms indicate means ± SD of three separate experiments each one tested in triplicate. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 versus untreated cells and °P < 0.05, °°P < 0.01, and °°°P < 0.001 versus LPS/ATP treated cells.