Figure 1.

Influence of error rates on de novo DNA synthesis. (A) The purity of gene synthesis products (yield of error-free clones) decreases exponentially with the length of the product synthesized. Error rates shown are 1 in 600 bp (blue) typical of conventional gene synthesis approaches, 1 in 1400 bp (red) (13), and 1 in 10 000 bp (yellow) as reported here. (B) The number of clones which must be sequenced to have a high (95%) probability of obtaining at least one which is error-free. The same three error rates as in (A) are indicated. Calculations are described in Supplementary Table A.