Figure 2.

Influence of inflammation on Ly6C and Ly6G expression in MDSC subsets. (a) Sorted CD11b⁺Ly6G^highLy6C^low G-MDSCs from the bone marrow of wild-type mice were cultured with LPS (100 ng/ml) in vitro for 3 h, collected, and assayed by FACS. Representative graphs of flow cytometry analysis are shown. (b) Gfi1:GFP knock-in mice were exposed to LPS (i.p. 10 mg/kg) or CLP surgery. Bone marrow, spleen, and blood cells were stained with fluorescence-conjugated antibodies after 3 h LPS and on days 1, 7, and 12 after CLP. M-MDSCs were gated as CD11b⁺Ly6G^lowLy6C^high and their purity was evaluated by Gfi1:GFP expression (graphs of bone marrow are shown). (c) Statistical analysis of flow cytometry data. Data are mean ± SEM of 5–8 mice per group.