Fig. 7.

Histological investigation of orbital sections and digitised analysis of retroorbital tissue: effects of novel peptides. The effects of peptide therapy on severity of retro-orbital fibrosis were evaluated in histological sections. The measurements were carried out in mice which had either received four weekly injections with vehicle (“Graves’—no therapy”) or of a peptide (1 mg/kg body weight), or in age-matched immunologically naïve mice. The mean total fibrosis volumes of each right and left orbita, as assessed by digitised image analysis of all sections, and consecutive integrations, are shown with SEM. N = 10 mice were investigated in each group. Differences between groups were tested by ANOVA, *p < 0.05 compared to the TSHR-immunised group treated with only NaCl. Also, results of TSHR-immunised mice differed significantly (p < 0.05) from healthy controls