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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mol Genet Metab. 2016 Dec 8;120(3):198–206. doi: 10.1016/j.ymgme.2016.12.002

Fig. 4.

Fig. 4

Thermal stability of wild-type CPS1 and E1034G mutant. (A) Remaining relative enzyme activity after incubation for 15 min at the indicated temperature for wild-type CPS1 in presence of MgATP (blue curve), MgATP + NAG (yellow curve) or MgATP + NCG (purple curve). (B) Relative fluorescence for wild-type CPS1 in presence of MgATP (blue curve), MgATP + NAG (green curve) or MgATP + NCG (brown curve) when temperature ramps from 298 K to 353 K. (C) Remaining relative enzyme activity after incubation for 15 min at the indicated temperature for the E1034G mutant in presence of MgATP (blue curve), MgATP + NAG (yellow curve) or MgATP + NCG (brown curve). (D) Relative fluorescence for the E1034G mutant in presence of MgATP (blue curve), MgATP + NAG (green curve) or MgATP + NCG (brown curve) when temperature ramps from 298 K to 353 K.