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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mol Genet Metab. 2016 Dec 8;120(3):198–206. doi: 10.1016/j.ymgme.2016.12.002

Fig. 5.

Fig. 5

Protection of CPS1 in the presence of NAG or NCG with MgATP. (A–B) Remaining relative activity for the wild-type CPS1 (A) and E1034G mutant (B) after incubation with elastase for 40 and 60 min in the presence of MgATP (brown curve), MgATP + NAG (blue curve) or MgATP + NCG (green curve). (C–D) Fluorescence signals for the wild-type CPS1 (C) and E1034G mutant (D) after incubation with elatase for 10 min in the presence of MgATP (brown dots), MgATP + NAG (blue dots) or MgATP + NCG (green dots). (E) Relative fluorescence for the M792I mutant in presence of MgATP (blue curve), MgATP + NAG (green curve) or MgATP + NCG (red curve) when temperature ramps from 298 K to 353 K. (F) Fluorescence signals for the M792I mutant after incubation with elastase for 10 min in the presence of MgATP (blue dots), MgATP + NAG (green dots) or MgATP + NCG (red dots).