Table 4.
Gene expression comparisons among young and old stressed and control workers
| Transcript | Treatment Effect |
Age Effect | Interaction Effect |
Average*
ΔCt ± S.D. |
Young | Old |
|---|---|---|---|---|---|---|
| DWV | F(2,65) = 2.0, p = 0.140 |
F(1,65) = 58.4,
p < 0.001 |
F(2,65) = 0.9, p = 0.408 |
Control: Varroa: Paraquat: |
−7.3 (± 5.6) −9.0 (± 6.1) −4.7 (± 4.4) |
−14.7 (± 2.4) −15.9 (± 3.8) −14.7 (± 3.6) |
| Catalase | F(2,70) = 2.4, p = 0.097 |
F(1,70) = 54.7,
p < 0.001 |
F(2,70) = 0.9, p = 0.690 |
Control: Varroa: Paraquat: |
−7.0 (± 2.1) −8.4 (± 1.2) −7.6 (± 2.3) |
−4.6 (± 1.2) −5.4 (± 1.1) −4.4 (± 0.8) |
| mGST1 | F(2,73) = 1.3, p = 0.266 |
F(1,73) = 19.7,
p < 0.001 |
F(2,73) = 0.0, p = 0.994 |
Control: Varroa: Paraquat: |
−5.6 (± 4.6) −6.5 (± 5.3) −4.8 (± 3.9) |
−1.9 (± 2.2) −2.7 (± 3.0) −0.9 (± 2.5) |
| Vg | F(2,66) = 0.4, p = 0.669 |
F(1,66) = 9.6,
p = 0.003 |
F(2,66) = 0.6, p = 0.567 |
Control: Varroa: Paraquat: |
−3.5 (± 3.7) −4.3 (± 1.6) −5.1 (± 2.7) |
−7.7 (± 3.7) −6.4 (± 5.7) −7.5 (± 3.7) |
| PPOact | F(2,60) = 0.2, p = 0.812 |
F(1,60) = 4.3,
p = 0.042 |
F(2,60) = 0.6, p = 0.526 |
Control: Varroa: Paraquat: |
−9.8 (± 3.8) −9.0 (± 4.4) −6.9 (± 12.8) |
−11.9 (± 2.4) −11.8 (± 3.0) −12.7 (± 3.8) |
| Hymenop- taecin |
F(2,60) = 0.6, p = 0.555 |
F(1,60) = 1.7, p = 0.204 |
F(2,60) = 0.3, p = 0.733 |
Control: Varroa: Paraquat: |
−6.6 (± 2.6) −7.6 (± 3.0) −6.6 (± 2.8) |
−6.1 (± 3.0) −6.2 (± 5.7) −4.4 (± 5.0) |
| SOD1 | F(2,59) = 2.5, p = 0.087 |
F(1,59) = 1.1, p = 0.289 |
F(2,59) = 0.1, p = 0.924 |
Control: Varroa: Paraquat: |
−1.7 (± 1.8) −2.8 (± 1.6) −2.5 (± 1.7) |
−1.5 (± 1.1) −2.3 (± 1.0) −2.1 (± 0.5) |
*
Sample sizes: N(young, control) = 13, N(young, paraquat) = 14, N(young, Varroa) = 7, N(old, control) = 23, N(old, paraquat) = 15, N(old, Varroa) =7.