Table 1.
LC-MS/MS settings and conditions
| LC-MS/MS settings and conditions | |||
|---|---|---|---|
|
Mass spectrometer settings | |||
| Curtain Gas | 15 | ||
| Ion Spray Voltage | −4500v | ||
| Dissolvation Temperature |
600°C | ||
| GS1 and GS2 | 20 and 40 respectively | ||
| Declustering potential | −110v | ||
| Collision energy (CE) for different MRM transitions | |||
| Succinyl-CoA | 866.2> 408 (CE −55ev) | ||
| D4-Succinyl-CoA | 870.2 > 408 (CE-55ev) | ||
| Malonyl-CoA | 852.2> 808 (CE −40ev) | ||
| Chromatography Gradient conditions | |||
| Time (min) |
Flow rate (ml/min) |
Solvent A%# |
Solvent B%# |
| 0 | 0.15 | 70 | 30 |
| 2 | 0.15 | 30 | 70 |
| 6.5 | 0.15 | 30 | 70 |
| 7.5 | 0.15 | 70 | 30 |
| 10 | 0.15 | 70 | 30 |
#
Solvent A, 10mM ammonium formate in acetonitrile/water (90/10, vol/vol); Solvent B, 10mM ammonium formate in acetonitrile/water (20/80, vol/vol)