Skip to main content
. 2004 Dec 1;18(23):2905–2915. doi: 10.1101/gad.1223004

Figure 2.

Figure 2.

JNK represses TNF-stimulated apoptosis. (A) Wild-type (WT) and Jnk-/- fibroblasts were incubated with 10 ng/mL TNF (6 h). The effect of the expression of ΔN-IκBα was examined. Apoptosis was investigated by measuring DNA fragmentation by ELISA. Wild-type fibroblasts were also incubated with TNF plus the protein synthesis inhibitor emetine (2 μM). The data shown are the mean ± SD of triplicate determinations. The data are representative of three independent experiments. (B) Wild-type (WT) and Jnk-/- fibroblasts incubated without and with 10 ng/mL TNF (6 h) in the presence of the protein synthesis inhibitor emetine (2 μM) were examined. The effect of the expression of ΔN-IκBα was examined. Apoptosis was investigated by measuring DNA fragmentation by ELISA. The data shown are the mean ± SD of triplicate determinations. The data are representative of three independent experiments. (C) Wild-type (WT) and Jnk-/- fibroblasts were incubated (6 h) without and with 10 ng/mL TNF plus 2 μM emetine. Nuclear DNA was stained with DAPI (blue). Apoptotic cells were detected by using a TUNEL assay (red).