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. 2004 Dec 1;18(23):2941–2951. doi: 10.1101/gad.1239304

Figure 2.

Figure 2.

E2F4 compensates for loss of E2F6 in E2F6-/- cells. (A) Chromatin immunoprecipitation assays for interaction of E2F6 and E2F4 with the mouse RR2, DHFR, and cdc6 promoters as cells progress through the cell cycle. Mouse embryo fibroblasts (wild-type [WT] or E2F6-/-) were harvested either at quiescence (Q) or 0, 6, 9, and 12 h following release from a hydroxyurea (HU) block and cross-linked with addition of formaldehyde as in Figure 1. (B) Nuclear and cytoplasmic protein extracts of wild-type (WT), E2F6-/-, or E2F4-/- cells. Cells were harvested either at quiescence (Q) or 0, 6, 9, and 12 h following a hydroxyurea block (HU). Cell extracts were resolved in an SDS acrylamide gel and assayed for the presence of mouse E2F4 protein by Western blotting with specific antibodies (see Materials and Methods).