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. 2017 Mar 13;10:68. doi: 10.3389/fnmol.2017.00068

Figure 4.

Figure 4

Metastasis-associated in colon cancer 1 (MACC1) acted as an oncogenic role in glioma tissue and GSCs. (A) MACC1 protein expression levels in nontumorous brain tissues (NBTs), low-grade glioma tissues (World Health Organization [WHO] I–II), and high-grade glioma tissues (WHO III–IV). Data are presented as the mean ± SD (n = 3, each group) *P < 0.05 vs. NBTs group. (B) MACC1 protein expression levels in U87, U251 and GSCs. Data are presented as the mean ± SD (n = 5, each group) *P < 0.05 vs. non-GSCs group. (C) Western blot assay was performed to detect the expression of MACC1 in GSCs treated with EMAP-II, TMZ or EMAP-II + TMZ. Data are presented as the mean ± SD (n = 5, each group) *P < 0.05 vs. Control group, **P < 0.01 vs. Control group, #P < 0.05 vs. EMAP-II group, ΔP < 0.05 vs. TMZ group. MACC1 knockdown induced GSCs autophagy. (D,E) The knockdown efficiency of MACC1 by shRNA were detected by qRT-PCR and Western blot assay. (F–H) Western blot analysis was performed to detect the expression of autophagy-related genes. Data are presented as the mean ± SD (n = 5, each group) *P < 0.05 vs. sh-NC group. Data are presented as the mean ± SD (n = 5, each group) *P < 0.05 vs. sh-NC group, **P < 0.05 vs. sh-NC group.