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. 2017 Mar 13;10:68. doi: 10.3389/fnmol.2017.00068

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Copyright © 2017 Zhou, Liu, Xue, Zheng, Liu, Ma, Li and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of miR-590-3p inhibited the expression of MACC1 by targeting its 3′-UTR. (A) Effect of miR-590-3p on the mRNA expression of MACC1 in GSCs. (B) Effect of miR-590-3p on the protein expression of MACC1 in GSCs. Data are presented as the mean ± SD (n = 5, each group) *P < 0.05 vs. pre-NC group, ^#P < 0.05 vs. anti-NC group. (C) The predicted miR-590-3p binding sites in the 3′-UTR region of MACC1 (MACC1-3′-UTR-Wt1 (Wt2)) and the designed mutant sequence (MACC1-3′-UTR-Mut1 (Mut2)) were indicated. (D) Luciferase activities were significantly reduced in human embryonic kidney (HEK) 293T cells co-transfected with MACC1-Wt1 (MACC1-Wt2) and pre-miR-590-3p, but not in in HEK 293T cells co-transfected with MACC1-Mut1 (MACC1-Mut2) and pre-miR-590-3p. Data are presented as the mean ± SD (n = 5, each group) *P < 0.05 vs. MACC1-Wt1 (MACC1-Wt2) + pre-NC group.