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. 2017 Mar 13;10:68. doi: 10.3389/fnmol.2017.00068

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Copyright © 2017 Zhou, Liu, Xue, Zheng, Liu, Ma, Li and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MACC1 mediated the effect of miR-590-3p in the combination treatment inhibited the malignant biological behaviors of GSCs via inducing autophagy. (A) Cell viability was detected by CCK8 assay to evaluate the effect of miR-590-3p and MACC1. (B) Cell migration and invasion of GSCs was measured by transwell assay to evaluate the effect of miR-590-3p and MACC1. (C–E) Western blot assay was performed to detect the expression of autophagy-related genes to evaluate the effect of miR-590-3p and MACC1. Data are presented as the mean ± SD (n = 5, each group) *P < 0.05 vs. anti-NC + sh-NC group, ^#P < 0.05 vs. anti-miR-590-3p + anti-NC group.