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. 2017 Mar 13;10:68. doi: 10.3389/fnmol.2017.00068

Figure 7.

Figure 7

EMAP-II in combination with TMZ induced GSCs autophagy through MACC1 inhibiting PI3K/AKT/mTOR signaling pathway. (A–E) GSCs were treated with EMAP-II, TMZ or EMAP-II + TMZ. Western blot assay was performed to detect the PI3K, Akt, mTOR, S6 and 4EBP signal molecules. (F–J) MACC1 knockdown inhibited the PI3K/AKT/mTOR pathway. Western blot analysis of the PI3K/AKT/mTOR pathways regulated by MACC1 in GSCs. Data are presented as the mean ± SD (n = 5, each group) *P < 0.05 vs. sh-NC group. (K–M) PI3K/Akt agonist IGF-1 partly blocked the effect of EMAP-II, TMZ or EMAP-II + TMZ on the expression of autophagy related genes. Data are presented as the mean ± SD (n = 5, each group) *P < 0.05 vs. Control group, **P < 0.01 vs. Control group, #P < 0.05 vs. EMAP-II group, ΔP < 0.05 vs. TMZ group, &P < 0.01 vs. EMAP-II + TMZ group.