FIGURE 5.

Improved tolerance to salt in the BdGF14d-overexpression plants. (A) The relative expression levels of BdGF14d in transgenic tobacco plants. The Ntubiquitin was used as internal reference. (B) Seven-day-old seedlings of WT, VC, and transgenic lines germinated on MS were transferred to 1/2 MS containing 100 mM and 150 mM NaCl to grow for a further 2 weeks. (C) The root lengths were measured and statistically analyzed. (D) Two-week-old seedlings growing on MS medium were planted in soil to grow under normal condition for 3 weeks and 500 mM NaCl solution was used to treat the seedlings for a further 35 days. (E) The respective survival rates were calculated. All the experiments were repeated three times. Bars are means (±SE); asterisks denote significant differences between WT and the transgenic lines (^∗P < 0.05; ^∗∗P < 0.01).